I Solation and Identification of P Rotease Producing B Acteria from S Oil

Protease producing bacteria was isolated from marine soil sample from Marakkanam Saltern, Tamil Nadu. Based on their morphology, it was identified as Pseudonomonas fluorescens biovar. The bacteria were inoculated in gelatin agar for the production of protease enzyme. The crude enzyme extract was purified by ammonium sulphate precipitation method to obtain 85% saturation. The protease enzyme activity of P. fluorescens biovar was 7.5 U/ml after 24 hours of incubation. The maximum extracellular protease production was observed at 37 C with a specific activity of 49.02 U/mg.